[Published online The Keio Journal of Medicine Vol.70, 35-43, by J-STAGE]
<Title:> RT-PCR Screening Tests for SARS-CoV-2 with Saliva Samples in Asymptomatic People: Strategy to Maintain Social and Economic Activities while Reducing the Risk of Spreading the Virus
<Author(s):> Junna Oba, Hiroaki Taniguchi, Masae Sato, Reika Takamatsu, Satoru Morikawa, Taneaki Nakagawa, Hiromasa Takaishi, Hideyuki Saya, Koichi Matsuo, Hiroshi Nishihara
<Abstract:> The year 2020 will be remembered for the coronavirus disease 2019 (COVID-19) pandemic, which continues to affect the whole world. Early and accurate identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is fundamental to combat the disease. Among the current diagnostic tests, real-time reverse transcriptase polymerase chain reaction (RT-qPCR) is the most reliable and frequently used method. Herein, we discuss the interpretation of RT-qPCR results relative to viral infectivity. Although nasopharyngeal swab samples are often used for RT-qPCR testing, they require collection by trained medical staff. Saliva samples are emerging as an inexpensive and efficient alternative for large-scale screening. Pooled-sample testing of saliva has been applied for mass screening of SARS-CoV-2 infection. Current policies recommend isolating people with borderline cycle threshold (Ct) values (35<ct <40),="" despite="" these="" ct="" values="" indicating="" minimal="" infection="" risk.="" we="" propose="" the="" new="" concept="" of="" a="" “social="" cut-off”="" value="" and="" risk="" stratification="" based="" on="" correlation="" with="" infectivity.="" also="" describe="" experience="" rt-qpcr="" screening="" saliva="" samples="" at="" our="" institution.="" it="" is="" important="" to="" implement="" scientific="" approach="" minimize="" viral="" transmission="" while="" allowing="" economic="" social="" activities="" continue.=""
<Keywords:> COVID-19, SARS-CoV-2, real-time polymerase chain reaction, saliva, mass screening
<URL:> https://www.jstage.jst.go.jp/article/kjm/70/2/70_2021-0003-OA/_html
<Title:> RT-PCR Screening Tests for SARS-CoV-2 with Saliva Samples in Asymptomatic People: Strategy to Maintain Social and Economic Activities while Reducing the Risk of Spreading the Virus
<Author(s):> Junna Oba, Hiroaki Taniguchi, Masae Sato, Reika Takamatsu, Satoru Morikawa, Taneaki Nakagawa, Hiromasa Takaishi, Hideyuki Saya, Koichi Matsuo, Hiroshi Nishihara
<Abstract:> The year 2020 will be remembered for the coronavirus disease 2019 (COVID-19) pandemic, which continues to affect the whole world. Early and accurate identification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is fundamental to combat the disease. Among the current diagnostic tests, real-time reverse transcriptase polymerase chain reaction (RT-qPCR) is the most reliable and frequently used method. Herein, we discuss the interpretation of RT-qPCR results relative to viral infectivity. Although nasopharyngeal swab samples are often used for RT-qPCR testing, they require collection by trained medical staff. Saliva samples are emerging as an inexpensive and efficient alternative for large-scale screening. Pooled-sample testing of saliva has been applied for mass screening of SARS-CoV-2 infection. Current policies recommend isolating people with borderline cycle threshold (Ct) values (35<ct <40),="" despite="" these="" ct="" values="" indicating="" minimal="" infection="" risk.="" we="" propose="" the="" new="" concept="" of="" a="" “social="" cut-off”="" value="" and="" risk="" stratification="" based="" on="" correlation="" with="" infectivity.="" also="" describe="" experience="" rt-qpcr="" screening="" saliva="" samples="" at="" our="" institution.="" it="" is="" important="" to="" implement="" scientific="" approach="" minimize="" viral="" transmission="" while="" allowing="" economic="" social="" activities="" continue.=""
<Keywords:> COVID-19, SARS-CoV-2, real-time polymerase chain reaction, saliva, mass screening
<URL:> https://www.jstage.jst.go.jp/article/kjm/70/2/70_2021-0003-OA/_html
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